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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Lysosome proteolysis analysis with DQ-BSA
Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.
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Astrocyte extraction from brain organoids V.2
Protocol for astrocyte extraction from brain organoids.
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Midbrain organoid differentiation in spinner flasks
Midbrain differentiation protocol using spinner flasks.
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Radiolabeled polyamine uptake in cells
This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.
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Fluorescently labeled polyamine uptake (via Flow Cytometry)
Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry. Watch an interview about this protocol with Mujahid Azfar, MSc.
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Evaluating endocytic rate in cells
Assess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
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Western blot protocol for detecting ATP10B in mouse/rat brain
This protocol describes the procedure for detection of ATP10B in rat and mouse brain tissue by Western blotting
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Microsomal membrane isolation from cell culture
This protocol describes microsomal membrane isolation from cell culture.
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Immunofluorescent staining with anti-GFP and anti-CD63 antibodies
This protocol describes immunofluorescent staining with anti-GFP and anti-CD63 antibodies.
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Cellular lipid uptake with flow cytometry readout
This protocol describes cellular lipid uptake with flow cytometry readout.
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Western blotting to detect ATP13A2 and ATP13A3
Protocol to detect ATP13A2 and ATP13A3 via Western blotting.
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