Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Lysosome proteolysis analysis with DQ-BSA

Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.

Protocol

Radiolabeled polyamine uptake in cells

This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.

 

Protocol

Fluorescently labeled polyamine uptake (via Flow Cytometry)

Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry. Watch an interview about this protocol with Mujahid Azfar, MSc.

Protocol

Evaluating endocytic rate in cells

Assess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.

 

Protocol

Western blotting to detect ATP13A2 and ATP13A3

Protocol to detect ATP13A2 and ATP13A3 via Western blotting.

 

Protocol

Western blotting

This protocol describes western blotting.

Protocol

Microsomal membrane isolation from cell culture

This protocol describes microsomal membrane isolation from cell culture.

Protocol

Western blot protocol for detecting ATP10B in mouse/rat brain

This protocol describes the procedure for detection of ATP10B in rat and mouse brain tissue by Western blotting

Protocol

Cell surface biotinylation

This protocol describes cell surface biotinylation.

Protocol

Cellular lipid uptake with flow cytometry readout

This protocol describes cellular lipid uptake with flow cytometry readout.

Protocol

Fixation and immunostaining protocol

Fixation and immunostaining protocol for tissue and cell culture.

Protocol

Western blot for tissue extract

Protocol for the detection of proteins by Western blot from tissue extract.