This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
HyDrop Bead Generation & PCR Barcoding v1.0
Protocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.
Teams
Themes
Protocol
Microfluidic Chip Production v1.1 V.2
Protocol for producing microfluidic chips used in HyDrop experiment.
Teams
Themes
Teams
Themes
Protocol
HyDrop-RNA v1.0
Step-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step.
Teams
Themes
Protocol
Single-cell Whole Genome Amplification (scWGA) of human post-mortem brain samples isolated by Laser Capture Microdissection (LCM)
This protocol uses Laser Capture Microdissection (LCM) technology on human post-mortem brain tissue slides with rapid Giemsa staining to isolate single nuclei for Single-cell Whole Genome Amplification (scWGA) in order to do low coverage (<1x) single-cell whole genome sequencing to detect mega-base somatic Copy Number Variations (CNVs).
Teams
Themes
Protocol
Manual isolation of nuclei from human brain using CellRaft device and single nucleus Whole Genome Amplification
Protocol for manual nuclear isolation from human brain tissue using Cell Raft device for single cell Whole Genome Amplification Protocol for manual nuclear isolation from human brain tissue using Cell Raft device for single cell Whole Genome Amplification
Teams
Themes
Protocol
Section 1: Enzymatic DNA Fragmentation (Manually)
This protocol details manual enzymatic DNA Fragmentation prior to Section 2: NGS library preparation for sequencing.
Teams
Themes
Protocol
Section 2: NGS library preparation for sequencing
This protocol details NGS library preparation for sequencing and should be performed after Section 1: Enzymatic DNA Fragmentation (Manually).
Teams
Themes
Protocol
Section 3: Libraries quality control (QC)
This protocol details quality control of libraries and should be performed after Section 2: NGS library preparation for sequencing.
Teams
Themes
Protocol
Targeted detection of SNCA CNVs in SOX10+ nuclei from oligodendrocytes containing alpha-synuclein inclusions isolated from human post-mortem brain
The protocol is optimized to detect SOX10, a nuclear oligodendrocyte marker, and alpha-synuclein inclusions, which are frequently retained at the perinucleus in MSA (the so-called Papp-Lantos inclusions). This protocol also describes its use in affected PD and MSA brain regions such as the putamen, substantia nigra (SN), and cerebellum.
Teams
Themes