This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Live-cell imaging; mitochondrial reactive oxygen species
This protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye (Thermo Fisher Scientific), a reduced non-fluorescent version of Mitotracker red that is fluorescent upon oxidation within mitochondria, which accumulates in mitochondria upon oxidation.
Teams
Themes
Protocol
SoRA microscopy protocol for imaging oligomers in human brain tissue
This protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope. The microscope used for this protocol was a commercial set up produced by 3i; however the techniques described can be used as general guidelines with alternative microscopes.
Teams
Themes
Protocol
Free-floating mouse brain immunohistochemistry
This protocol enables immunohistochemical staining of murine tissue with superior penetration of the tissue by the reagents due to the free-floating approach. Watch an interview about this protocol with Jonathan Breiter, MSc.
Teams
Themes
Protocol
Single-molecule antibody slides for fluorescence microscopy
This protocol describes how to create single-molecule antibody slides for fluorescence microscopy.
Teams
Themes
Teams
Themes