ICC confocal images: Protein aggregation and calcium dysregulation are the earliest hallmarks of familial Parkinson’s disease in human midbrain dopaminergic neurons
By savannah onOur differentiation paradigm generates an efficient model for studying disease mechanisms in PD, and highlights that protein misfolding to generate intraneuronal oligomers is one of the earliest critical events driving disease in human neurons, rather than a late-stage hallmark of the disease.
Motor learning selectively strengthens cortical and striatal synapses of motor engram neurons Dataset
By Emma Sherrell onThis upload includes 1) ImageJ macros / scripts for minor automated cell counting for brain slides with immunostaining and 2) MATLAB functions / scripts for minor automated spine location cluster analysis (nearest neighbor distance / index).
Source data for “Ca2+ channels couple spiking to mitochondrial metabolism in substantia nigra dopaminergic neurons”
By Emma Sherrell onSource data for publication: "Ca2+ channels couple spiking to mitochondrial metabolism in substantia nigra dopaminergic neurons."
Day2023 dataset
By Emma Sherrell onDatasets for all figures 1-8 in: GABAergic regulation of striatal spiny projection neuron excitability depends upon their activity state Image is taken from Fig 1B inset and illustrates RiboTag-eGFP in SPNs of the striatum.
Day2023 supplementary dataset
By Emma Sherrell onDatasets for all supplementary figures S1-4 in GABAergic regulation of striatal spiny projection neuron excitability depends upon their activity state (2023).
Characterizing dysbiosis of the Parkinson’s disease gut microbiome using shotgun metagenomics
By Emma Sherrell onTo characterize the dysbiosis at species, gene family, and metabolic pathway resolution, the authors enrolled 490 PD and 234 age and geographically matched neurologically healthy individuals, collected stool and metadata, and conducted deep shotgun metagenomic sequencing of fecal DNA. Sequences and metadata are provided here.
CryoEM structures of amplified alpha-synuclein fibril class A type I with extended core from DLB case I
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli BL21(DE3) Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class A type I with compact core from DLB case III
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class A type I with extended core from DLB case VII
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class A type I with extended core from DLB case X
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structures of amplified alpha-synuclein fibril class B type II with extended core from DLB case I
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli BL21(DE3) Mutation(s): No
CryoEM structures of amplified alpha-synuclein fibril class B mixed type I/II with extended core from DLB case II
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structures of amplified alpha-synuclein fibril class B type I with compact core from DLB case III
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class B type I with extended core from DLB case VII
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class B type II with extended core from DLB case VII
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class B type I with extended core from DLB case X
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
Alpha-synuclein fibril from spontaneous control
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
Examining the brain’s response to intestinal permeability and inflammation in the dextran sulfate sodium-induced colitis model
By Emma Sherrell onFrom the brain, cortex, hippocampus, ventral midbrain, and striatum were taken to examine both global and regionally-specific transcriptomic perturbances that emerge in response to gut leakiness. All tissues were assayed with high-throughput RNA sequencing.
Raw band intensity values for ATG3 WT or Mutants in vitro LC3 lipidation
By Emma Sherrell onRaw band intensity values used for the quantification of in vitro LC3 lipidation results (Fig4C) in Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore.
Raw band intensity values for Halo-LC3B
By Emma Sherrell onRaw band intensity values used for the quantification of percentage autophagy levels.