H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 FAM134A-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A.
H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-REEP5
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1 and expressing the indicated Keima-REEP5 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-RAMP4
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1 and expressing the indicated Keima-RAMP4 ER-phagy flux reporter.
H9 ES AAVS1-NGN2 CCPG1-/-
By savannah onCell Line: ES cells for making iNeurons lacking the ER-phagy receptor CCPG1.
A549 PPM1H-BromoTag (CVCL_C8YV)
By savannah onCell Line: PPM1H-BromoTag CRISPR/CAS9 A549 knock-in cell line generated from parental A549 cells (ATCC A549 CCl-185) by introducing a BromoTag linked to the C-terminus of PPM1H using CRISPR/Cas9 gene-editing technology.
HEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/-
By savannah onHEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/- PubMed=37757899.
pLP201_pCCL_EFSp_GreenLantern_WPRE
By Emma Sherrell onpLP201_pCCL_EFSp_GreenLantern_WPRE fluorescent reporter plasmid.
pLP202_pCCL_EFSp_mScarlet_WPRE
By Emma Sherrell onpLP202_pCCL_EFSp_mScarlet_WPRE fluorescent reporter plasmid.
Testing AAVrg-hSyn-mCherry in the spinal cord of Rhesus Macaques
By Emma Sherrell onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the spinal cord. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the spinal cord).
Video tutorials for Curtain and Curtain-PTM
By Emma Sherrell onVideo tutorials to assist users of Curtain and Curtain-PTM proteomic data analysis web tools (described in https://www.biorxiv.org/content/10.1101/2023.07.25.550405v1).
UniProtExtractR
By Emma Sherrell onThe app features interactive frequency tables that globally summarize both the original UniProtKB input query as well as the extracted/changed entry values.
R scripts for analysis of publication “Sex distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson Disease”
By Emma Sherrell onR scripts for analysis of publication "Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease."
Lentiviral vector plasmid for overexpression of ATP10B (E993A)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (E993A) validated for use in cell culture.
Lentiviral vector plasmid for overexpression of ATP10B (I1038T)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (I1038T) validated for use in cell culture.
Lentiviral vector plasmid for knock down of ATP10B (miR5 Hs)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for knock down of ATP10B (miR5, Hs) validated for use in cell culture.
Testing AAVrg-hSyn-mCherry in the Putamen of Rhesus Macaques
By Emma Sherrell onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the putamen. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the putamen).
pCMV-pOTC-GFP
By savannah onExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.