Protocol Archive

This protocol describes a multi-fiber photometry approach to track dopamine release in mice with high spatial and temporal resolution in multiple locations in the brain simultaneously.

This protocol includes the steps for targeted optogenetic manipulation using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.

This protocol includes the steps for fiber array imaging setup and acquisition using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.

This protocol includes the stereotaxic viral injections and array implantation steps using a micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.

This protocol includes the micro-CT scanning and fiber localization steps for a micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.

This protocol includes immunohistology steps performed in mouse brain sections to confirm viral expression and stain for neurons after whole-brain micro-CT scanning and contrast agent soaking procedure.

This protocol includes the steps for imaging in freely moving mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.

This protocol includes the steps for imaging in head-fixed mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.

This protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo

This protocol details the extraction of brain infiltrating leukocytes (BILs).

A protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM).

Protocol for making Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices.

Protocol for intracranial viral vector injections in dorsal or ventral striatum in mice is outlined. Steps are generalizable but require optimization for injection coordinates and virus concentration.

The protocol outlines the injection of α-Synuclin PFF and monomer into the mouse brain, followed by the preparation of acute slices from mice for whole-cell patch clamp recordings.

Protocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.

This collection of protocols describes different behavioral assays performed in mice, including grip strength test, pole test, rotarod, open field, and nose poking learning task.

This protocol details the DAB immunohistochemistry staining for stereological analysis of 40 µM slices cut with cryostat and stored in antifreeze.

This protocol details the immunohistochemistry of free-floating slices.

This protocol details the Perfusion and Fixation for cryostat slicing for HHC.

This protocol details the purification of BNIP3-GST.