Protocol Archive

This is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.

Holding ACSF is used for incubating brain slices before cell-attached or whole-cell electrophysiological recordings of neurons.

Recording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.

This is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).

The authors describe imaging, analysis, and cell counts of coronal sections of mouse substantia nigra and ventral tegmental area labeled with Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).

The authors describe preparing and labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for probes targeting the genes Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).

Plasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson's disease and its pathophysiology.

Purification of total RNA from cells using spin technology.

Interest in the role of cellular glycosphingolipids (GSLs) in health and disease led the authors to develop a sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.

This protocol describes the methods of using a custom 3D printed stereotaxic frame to acquire MRI and CT scans for nonhuman primates in detailed steps.

This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect green fluorescent protein (GFP).

This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.

This protocol describes the free-floating multiplexed immunofluorescent staining protocol to ascertain levels of tau and phospho-tau in mouse tissue from transplanted human iPSC cells carrying different PD-related mutations.

This protocol describes the coupling of rabbit monoclonal TMEM192 antibody (Abcam recombinant Anti-TMEM192 antibody , BSA and Azide free, ab232600) to MyOne™ Epoxy Dynabeads™ (Invitrogen™, 34001D). The coupled beads generated using this protocol can be used for the isolation of untagged lysosomes from cells and tissues.

A sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.

Protocol for neuronal activation of the enteric nervous system.

Protocol for immunophenotyping peripheral blood immune cells.

Protocol to assess cellular phenotypes by multiparametric flow cytometry panels containing markers to identify cell types and activation states.

Protocol for rat brain processing to perform parallel histological analysis in one hemisphere and high dimensional flow cytometry in the other.

This protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal after injection.