Tail suspension test to assess depression/anxiety behavior in parkinsonian mice
By savannah onThe Tail Suspension Test is a mouse behavioral paradigm measuring behavioral despair or “depression-like” behavior and learned helplessness.
Transcardiac perfusion of mouse for brain tissue
By savannah onTranscardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum
By savannah onThis protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum.
Striatal cannula window implantation
By savannah onThis protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.
Stereotactic viral injection into subthalamic nucleus in mice
By savannah onThis protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery. This protocol has been adapted from Maite Azcorra's protocol for midbrain viral injections.
Sectioning of mouse brain by microtome
By savannah onThis protocol describes how to use the microtome to prepare and slice mouse brain sections for Immunohistochemistry.
Sectioning of mouse brain by cryostat
By savannah onThis protocol describes how to use the cryostat to prepare and slice mouse brain sections for Immunohistochemistry.
Rotarod Test to assess motor coordination in a mouse parkinsonian model
By savannah onThis protocol evaluates motor coordination in the rotarod assay in parkinsonian mice.
Pole Test to assess motor coordination in parkinsonian mice
By savannah onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Open Field Test to assess motor coordination in a mouse parkinsonian model
By savannah onThe Open Field task is a simple sensorimotor test used to determine general activity levels, gross locomotor activity, and exploration habits in rodent models of CNS disorders.
Isolation and processing of embryonic and postnatal brains
By savannah onThis protocol describes: -Harvesting embryos from females from embryonic day 10 to embryonic day 18 -Brain isolation of embryonic (e10-e18) and postnatal brains (p0-p30) -Processing and freezing of embryonic and postnatal brains.
Immunofluorescence staining for postmortem mouse brain tissue
By savannah onThis is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
Genotyping mice from ear clips
By savannah onThis protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
Endosome isolation
By savannah onSubcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Create mask for axonal quantification analysis with FIJI
By savannah onThis protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
Catalepsy test (Bar test)
By savannah onThe catalepsy test (bar test) was developed to test motor coordination and motor impairments.
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
By savannah onThis behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Acute striatal or midbrain fiber photometry in head-fixed mice
By savannah onWhile this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
Free-floating mouse brain immunohistochemistry
By savannah onThis protocol enables immunohistochemical staining of murine tissue with superior penetration of the tissue by the reagents due to the free-floating approach. Watch an interview about this protocol with Jonathan Breiter, MSc.