Protocol Archive

This protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.

Procedures for implanting and testing CFET devices in rats are described.

Methods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.

Methods for immunofluorescent staining of brain tissue with indwelling electrodes.

This is the protocol for immunofluorescent staining for neuronal marker MAP2.

This is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.

Methods to cut and retain implanted carbon fiber electrodes in brain tissue.

The Y-maze is a quick and easy way to evaluate a rodent’s spatial working memory by measuring its ability to effectively explore a Y-shape maze.

Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.

This is a protocol that describes how to use antibody staining to detect cellular epitopes by immunofluorescence microscopy.

This protocol describes an immunoprecipitation method to detect ubiquitination on a protein of interest.

Protocol for AAV production and administration to mice.

Protocol for neuronal imaging.

Protocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.

Protocol for metagenomic analysis from mouse fecal pellets.

Expansion microscopy is a super-resolution imaging technique that uses expandable hydrogels to increase the physical distance between fluorophores from fixed cells on coverslips. By isotropically expanding samples, the traditional wavelength-limited confocal microscopy imaging techniques can be improved upon ~4X in resolution. Here, the authors use expansion techniques developed previously by others to confocal image TMEM55B and RILPL1.

This is the protocol for determining neuromelanin concentration and data.

Protocol for metabolomic analysis from mouse fecal and cecal samples.

This is a protocol involving chemical oxidation and reduction methods followed by high-performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.

Protocol for proteomic analysis from mouse cecal samples.