Protocol Archive

Assay to determine LRRK2 protein binding to microtubules.

Protocol for immunofluorescent staining for LRRK2 in cultured cells using the MJFF2 (c41-2) antibody.

We developed a method for assessing mitochondrial clearance in primary hippocampal neurons.

A protocol for rapidly purifying Golgi across various cell lines and tissues without any modification. The Golgi purified using this method are highly enriched, intact, contaminant-free and, depending on solubilisation buffer, could be used for various downstream applications, such as proteomics and metabolomics.

This protocol details about intracellular cytokine (ICS) staining.

Protocol for expressing LRRK1 and LRRK2 in insect cells.

This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.

This protocol describes immunohistochemical staining of fixed brain sections.

Imaris intensity-based protocol for Colocalization analysis.

Image quantification.

LC3 lipidation on GUVs.

GUV preparation for membrane tube assay application

This protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitement and LC3 lipidation with PE. Screen reader support enabled.

GST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.

This protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for PRoBaND / Tracking Parkinson's describing clinical data and outcomes with respect to GBA status.

This protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.

Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs

This protocol details the expression and purification protocol of WIPI2d.

This protocol details the expression and purification protocol of GST-TAX1BP1.

This protocol details the expression and purification of GST-mCh-FYVE.