Protocol Archive

This protocol details how to monitor Clusterin binding to the very low-density lipoprotein receptor (VLDLR) by enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al., 2014.

This protocol details how to efficiently label protein aggregates at the N-terminal using Amyloid beta as example.

Harvesting and culturing of peritoneal macrophages and ex vivo stimulation with IFNy.

This protocol details how to efficiently monitor α-Synuclein aggregation by thioflavin-T fluorescence in a plate reader.

This protocol details how to purify the fusion protein GFP-Clu-tails from Escherichia coli.

This protocol details how to efficiently purify the fusion protein Maltose binding protein (MBP)-Clu-tail (204-238) from Escherichia coli.

In this protocol, the authors describe the preparation and maintenance of rat organotypic cultures (cortico-striatal) for AAV infection and imaging of the extracellular matrix.

Protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons. This protocol recapitulates the activation of developmental pathways known to be important for mDA neuron development and mimics key sequences of events taking place in human midbrain development.

This protocol details how to efficiently purify low-density lipoprotein (LDL) receptor-related protein associated protein 1 (LRPAP1 or RAP) from Escherichia coli.

STC-1 passaging and PD-associated microbe stimulation protocol.

SOP for RT (Reverse Transcription) Promega kit.

SOP for immune cells isolation mouse adult brain with CD45 beads.

This protocol is to make carbon-fiber microelectrode (CFM) for electrochemical recordings of monoamines in ex-vivo mouse brain slices. This protocol is optimized for the detection of dopamine from 300 µm coronal striatal sections, using a Miller voltammeter.

SOP for DSS kinetics studies.

SOP for mesoscale discovery assays.

SOP for flow cytometry after DSS-induced gut and brain injury.

SOP for TriZOL RNA and protein extraction.

SOP for BCA protein assay and western immunoblotting.

Quantitative PCR, 384 well format.

SOP for LBP ELISA after DSS-induced injury.