Stereotaxic injections
By savannah onStereotaxic injections for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.
Cellular lipid uptake with flow cytometry readout
By savannah onThis protocol describes cellular lipid uptake with flow cytometry readout.
Immunofluorescent staining with anti-GFP and anti-CD63 antibodies
By savannah onThis protocol describes immunofluorescent staining with anti-GFP and anti-CD63 antibodies.
Regular maintenance of human pluripotent stem cells
By savannah onThis protocol describes the regular maintenance and passaging human iPSCs.
Microsomal membrane isolation from cell culture
By savannah onThis protocol describes microsomal membrane isolation from cell culture.
Brain slice physiology and optogenetics
By savannah onThis protocol describes Chu Lab's standard external solutions for electrophysiology.
Brain slice preparation for electrophysiology recording
By savannah onThis protocol describes brain slice preparation for electrophysiology recording.
Immunohistochemistry Protocol – Chu Lab (Wichmann)
By savannah onThis protocol describes immunohistochemistry.
Confocal imaging and digital image analysis
By savannah onThis protocol describes confocal imaging on a Nikon A1R confocal microscope and digital image analysis.
Quantification of TH immunoreactivity
By savannah onThis protocol describes the quantification of TH immunoreactivity.
SoRA microscopy protocol for imaging oligomers in human brain tissue
By savannah onThis protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope. The microscope used for this protocol was a commercial set up produced by 3i; however the techniques described can be used as general guidelines with alternative microscopes.
Quantifying the LAMP1 positive puncta
By savannah onThis protocol describes quantifying the LAMP1 positive puncta.
Nuclear cytoplasmic fractionation
By savannah onThis protocol describes nuclear cytoplasmic fractionation.
Native gel lipid binding assay
By savannah onThis protocol details how to perform a native gel lipid binding assay to evaluate the number of lipids bound per molecule of 3xFLAG-SHIP164Δ901-1099.
Molecular cloning of SHIP164 plasmids for expression in mammalian cells
By savannah onThis protocol is to help with the molecular cloning of SHIP164 and the sequences of other proteins.