Protocol Archive

This protocol uses the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher, Cat# 84868).

TMT labeling.

Preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.

Mitochondrial isolation and quantification from HeLa cells.

Protocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.

Protocol for preparation of HeLa cell lysates for immunoblot analysis.

Induction of starvation stress using EBSS in HeLa cells.

Mitophagy induction in HeLa cells using Difereprone (DFP).

Mitophagy induction in HeLa cells using Oligomycin/Antimycin A.

Protocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.

Salivary collection participant instructions using OMNIgene ORAL OM 501 device.

This protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.

While this protocol is focused on injections of AAV viruses into the midbrain, it can be easily modified to inject into other brain regions or other viruses.

The Tail Suspension Test is a mouse behavioral paradigm measuring behavioral despair or “depression-like” behavior and learned helplessness.

Transcardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.

This protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum.

This protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.

This protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery. This protocol has been adapted from Maite Azcorra's protocol for midbrain viral injections.

This protocol describes how to use the microtome to prepare and slice mouse brain sections for Immunohistochemistry.

This protocol describes how to use the cryostat to prepare and slice mouse brain sections for Immunohistochemistry.