Protocol Archive

This protocol details aggregated aSyn Dot Blot assay.

This protocol details the term ‘neuron reconstruction’ which is used here to refer to the process of delineating the different components of neurons (soma, axon, dendrites, and dendritic spines) using light microscopy, to create a geometric model of these cells for subsequent quantitative analyses of aspects of their morphology.

In this protocol, the authors detail the steps to perform ex-vivo brain slice electrophysiology and optogenetic stimulation.

This protocol describes the steps for immunostaining and confocal imaging of ex vivo slices following an electrophysiological experiment.

This protocol was used to analysis glucosylceramide and glucosylsphingosine levels in mouse brains and liver. It was also described in a previous publication (Mol Cell Neurosci. 2020 Jan:102:103451, doi: 10.1016).

This protocol is about collecting mouse tissues for immunohistochemistry or biochemistry experiments. While it is mainly used for brain tissues, it also can be used for other visceral tissues.

This protocol has been used for high throughput quantification of immunohistochemistry data of mouse brain sections.

Micro-PET CT procedures for brain imaging of rats.

Protocol for the detection of proteins by Western blot from tissue extract.

This protocol details the siRNA knockdown in mouse embryonic fibroblasts (MEFs) for PINK1, but applicable for any other target.

This protocol details the extraction of RNA and PCR analysis.

Synapse function and gene enrichment analysis.

This protocol details the passaging and seeding mouse embryonic fibroblasts (MEFs) for experiments.

This protocol details the preparation of free floating coronal mouse brain sections.

This protocol details the mouse stereotaxic injections into the mouse brain.

Using the Rubicon RH - Rab7 crystal structure as a template (6wcw).

Labels sites of autophagosome generation.

Following treatment with antibodies/genetic methods of introducing fluorescent tags.

1:1 binding of Rubicon and Rab7.

Production of pseudotyped virus an subsequent transduction.