Constant potential amperometry in vitro
By Julia Julia Leonard onProtocol for constant potential amperometry recordings in vitro of evoked dopamine release in the rat striatum.
Rotarod test
By Julia Julia Leonard onProtocol to assess motor coordination and balance by using an accelerating rod.
Open field test
By Julia Julia Leonard onThe open field test is a simple test to evaluate general motor and explorative behavior in rodents.
Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)
By Julia Julia Leonard onThis protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).
Quantification of area and optical density of intracellular neuromelanin with Image J
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with Image J.
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections
By Julia Julia Leonard onProtocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Quantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic prioritization and hit validation
By Emma Sherrell onThis protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
In vivo carbon fiber electrode thread (CFET) implantation and testing procedures
By Emma Sherrell onProcedures for implanting and testing CFET devices in rats are described.
In vitro FSCV testing of carbon fiber electrodes to characterize functional operation in dopamine detection
By Emma Sherrell onMethods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.
Immunohistochemistry for carbon fiber thread electrodes
By Emma Sherrell onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By Emma Sherrell onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Human neuroblastoma cell line SH-SY5Y culturing
By Emma Sherrell onThis is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
Histology and retention of implanted carbon fiber thread electrodes in fixed tissue
By Emma Sherrell onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
Y-Maze protocol
By Julia Julia Leonard onThe Y-maze is a quick and easy way to evaluate a rodent’s spatial working memory by measuring its ability to effectively explore a Y-shape maze.
Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents
By Julia Julia Leonard onProtocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.
Immunostaining
By Julia Julia Leonard onThis is a protocol that describes how to use antibody staining to detect cellular epitopes by immunofluorescence microscopy.
Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
By Julia Julia Leonard onThis protocol describes an immunoprecipitation method to detect ubiquitination on a protein of interest.
Virus production and administration
By Julia Julia Leonard onProtocol for AAV production and administration to mice.
Tissue preparation, immunohistochemistry, imaging, and quantification
By Julia Julia Leonard onProtocol for neuronal imaging.