Protocol Archive

This protocol describes nuclear cytoplasmic fractionation.

This protocol details how to perform a native gel lipid binding assay to evaluate the number of lipids bound per molecule of 3xFLAG-SHIP164Δ901-1099.

This protocol is to help with the molecular cloning of SHIP164 and the sequences of other proteins.

This protocol details how to perform a lipid transfer assay with 3xFLAG-SHIP164Δ901-1099.

This protocol describes iPSC differentiation into microglia.

This protocol describes iPSC differentiation into macrophages.

This protocol describes the fixation and staining of cultured cells in paraformaldehyde solution.

This protocol describes the preparation from cell lysate from cultured cells and immunoblotting procedure.

This protocol is to help with generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells.

This protocol is to help with the imaging analysis of ectopic receptor localization and endosome size.

This protocol describes DQ-BSA quantification.

This protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.

This protocol details how to prepare cryo-EM samples of 3xFLAG-SHIP164Δ901-1099.

This protocol is to help with the maintenance, transfection, immunocytochemistry, and imaging of adherent mammalian cells.

This protocol describes the isolation of bone marrow derived macrophages.

This protocol details the procedure of Correlative Light Microscopy and Electron Microscopy (CLEM) with 3D Focus Ion Beam Scanning Electron Microscopy (FIBSEM) technique in Zeiss Crossbeam 550 FIBSEM system.

Mass spectrometry-based proteomics and phosphoproteomics are highly sensitive and un-biased techniques to study the proteome and phosphoproteome at a global scale. Using our protocols, we routinely identify and quantify >35,000 phosphosites and ~10,000 protein groups.

The authors present a method to identify the same brain region in human postmortem tissue to evaluate ciliary status. The striatum is identified morphologically and also by immunofluorescence microscopy using anti-DARPP-32 antibodies.

This protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.

PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems. https://www.embopress.org/doi/full/10.15252/embr.202256399