Protocol Archive

This collection contains protocols which describe the standard procedure of culturing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).

This protocol describes the thawing of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.

This protocol describes the standard procedure of thawing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).

This protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex.

This protocol describes a standard procedure for subcloning of genotype-confirmed human pluripotent stem cells (hPSCs).

Mouse transcardiac perfusion protocol and storage.

The intent of this standard operating procedure (SOP) is to describe procedures for mouse stereotaxic intracranial injection surgery.

This collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way. This collection follows nucleofection of hPSCs as described in detail in the collection “Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs.” dx.doi.org/10.17504/protocols.io.b4qnqvve

This protocol describes the experimental procedure used to measure single cell survival rates post nucleofection of human pluripotent stem cells (hPSCs).

This protocol describes a standard procedure for seeding nucleofected human pluripotent stem cells (hPSCs) in 96-well plates using limited dilution. This protocol follows nucleofection of hPSCs as described in detail in the collection “Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs.” dx.doi.org/10.17504/protocols.io.b4qnqvve

This protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.

This protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).

This protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.

This protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.

This protocol describes the standard procedure for preparing mRNA to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.

This protocol describes the procedure or preparing MEF-cultured human pluripotent stem cells (hPSCs) for the delivery of plasmids, mRNA, or ribonucleoprotein (RNP) using nucleofection.

This protocol describes the standard procedure preparing feeder-free human pluripotent stem cells (hPSCs) for nucleofection.

This protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.

This is Leschziner's Lab updated protocol for making cryo-EM grids for LRRK2 RCKW. This protocol, when using lower protein concentration, results in better monomer and dimer formation than the old protocol.

This protocol describes the standard procedure for staining pluripotency markers, e.g., OCT4, SSEA4, alkaline phosphatase, and etc. on human pluripotent stem cells (hPSCs).