Protocol Archive

Protocol for stereotaxic rat brain surgery for Substantia Nigra targeting.

Protocol for immunochemistry on paraffin sections.

Protocol for intracellular neuromelanin quantification.

Protocol for rat brain processing for histological analyses.

Protocol for immunofluorescence on paraffin sections.

Protocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method.

Protocol for cDNA synthesis.

Protocol for stereologic count with StereoInvestigator.

Protocol for gene expression analysis by quantitative Real-Time PCR (qPCR).

Protocol for RNA and protein extraction from bulk dissections.

The genotyping is used to identify if mice are heterozygote (hetero) or Wild-Type (WT). The aim of the work is to validate the digestion method, and PCR program, the PCR primers, and the interpretation of the results. Associated with publication: doi: 10.1093/brain/awx221.

Coated coverslips provide a nourishing adherent surface for cell culture. This protocol provides step by step instruction on how to coat coverslips for cell culture.

Perfusion-fixation procedure for adult rhesus monkeys.

Midbrain differentiation protocol using spinner flasks.

This protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye (Thermo Fisher Scientific), a reduced non-fluorescent version of Mitotracker red that is fluorescent upon oxidation within mitochondria, which accumulates in mitochondria upon oxidation.

Protocol for astrocyte extraction from brain organoids.

Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.

Protocol for Light microscopy immunoperoxidase staining.

Midbrain organoid generation from mfNPC.

This protocol describes how to express and purify human NDP52 and OPTN S177D S473D. The same procedure can be applied to purify wild type OPTN.