Protocol Archive

This protocol details the procedure of mito-Keima assay to assess mitophagy.

This protocol details the procedure of immunofluorescence assay (IFA).

This protocol describes how to express and purify human TBK1 tagged N-terminally with eGFP.

This protocol details the procedure of generation of CRISPR constructs.

This protocol describes the expression and purification of ULK1 Complex wt or K46I mutant.

This protocol details the procedure of determination of edits in CRISPR-edited cell lines by sequencing.

Lyso-IP is a purification method of lysosomes, which can then be used for proteomic analysis or for metabolomic analysis. The Lyso-IP is adapted from a previous reported method. Here we also describe processing steps using proteomics after lysosome purification in the context of HeLa cells lacking the GRN gene.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

A general protocol for immunological detection by Western blotting MTOR, MTOR (pS2448), ULK1, ULK1 (pS757), p70S6K, p70S6K (pT389), SQSTM1, CALCOCO2, MAP1LC3B, GABARAP, TFEB, TFE3, PGRN, HSP90, and PCNA.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.

This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.

This protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.

This protocol describes the restriction cloning strategy.

This protocol describes the procedure of plunge freezing for subsequent cryo-electron tomography.

This protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.

A protocol for sample preparation for LC-MS analysis of whole cell lysates and for lysosomal and endosomal fractions purified by Lyso-IP and Endo-IP.