Protocol Archive

LC3 lipidation on GUVs.

GUV preparation for membrane tube assay application

This protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitement and LC3 lipidation with PE. Screen reader support enabled.

GST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.

This protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for PRoBaND / Tracking Parkinson's describing clinical data and outcomes with respect to GBA status.

This protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.

Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs

This protocol details the expression and purification protocol of WIPI2d.

This protocol details the expression and purification protocol of GST-TAX1BP1.

This protocol details the expression and purification of GST-mCh-FYVE.

Expression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.

Expression and purification of GST-tagged ATG13/ATG101 constructs.

This protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.

There are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.

This protocol describes the design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics.

This protocol describes the steps for making implants used for deep brain stimulation (DBS) in rodents.

Protocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.

Setting crystal trays.

Setting crystal trays.

This protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).