Protocol Archive

This protocol describes how to express and purify GST-WIPI1/WIPI2d/WIPI3/WIPI4.

This protocol describes how to express and purify mCh-WIPI2d-IDR (364-426aa).

This protocol describes how to express and purify mCherry-WIPI2d/WIPI3.

This protocol describes how to express and purify Lambda Protein Phosphatase.

Methods for the generation and maintenance of transgenic tyrosinase mice.

This protocol describes how to express and purify CK2 kinase complex.

In situ hybridization protocol with DIG-labelled riboprobes.

This protocol describes how to express and purify SRC kinase.

To assess local effects of nomifensine (DAT and NET inhibitor) on striatal DA release in microdialysis experiments.

Protocol for the clarification of whole mouse brains to allow neuromelanin visualisation by Optical Projection Tomography.

This protocol describes how to express and purify GFP-FIP200 (CTR).

Protocol for general locomotion in rodents using open field arenas and Ethovision video tracking. Purpose: Assess cognitive, spontaneous motor activity, and anxiety. Note that there are many variations of this test. The authors perform this test during the mouse's dark cycle (i.e. their active phase).

This protocol details the purification of FKBP8-GST.

This protocol details the purification of CCPG1-GST.

This protocol details the purification of BCL2L13-GFP.

Here, the authors describe a method based on an AI deep learning algorithm (Aiforia) for the purpose of accurately quantifying the number and location of neurons expressing alpha-synuclein throughout pre-defined regions of interest (cortical and sub-cortical locations) in the non-human primate brain.

This protocol details the purification of FUNDC1-GFP.

Slice preparation for electrophysiology.

This protocol details the purification of BNIP3-GFP.

Behavioral assay to measure fear learning and memory in mice.