SOP for flow cytometry after DSS-induced gut and brain injury
By Emma Sherrell onSOP for flow cytometry after DSS-induced gut and brain injury.
SOP for TriZOL RNA and protein extraction
By Emma Sherrell onSOP for TriZOL RNA and protein extraction.
SOP for BCA protein assay and western immunoblotting
By Emma Sherrell onSOP for BCA protein assay and western immunoblotting.
SOP for LBP ELISA after DSS-induced injury
By Emma Sherrell onSOP for LBP ELISA after DSS-induced injury.
SOP for cDNA synthesis by RT (Reverse Transcription) Promega kit
By Emma Sherrell onSOP for cDNA synthesis by RT (Reverse Transcription) Promega kit.
Very low-density lipoprotein receptor (VLDLR)-C-tag purification from HEK293E cells
By Emma Sherrell onThis protocol details how to purify recombinant very low-density lipoprotein receptor (VLDLR)-C-tag protein from HEK293E cells.
Formation and isolation of Clu phospholipid particles
By Emma Sherrell onThis protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).
N_terminal protein labeling
By Emma Sherrell onThis protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.
Clusterin cellular uptake assay
By Emma Sherrell onThis protocol details how to efficiently monitor Clusterin and Clusterin/Substrate uptake in different cell types like HEK293T, iNeurons, and iMicroglia.
Open field test for mice
By Emma Sherrell onThe open field test is a classical test for the quantification of locomotor activity and anxiety-like behaviors in laboratory animals. This protocol is optimized for mice, and it includes the automatic analysis of their movement by Biobserve Viewer.
Parallel beam test for mice
By Emma Sherrell onThe parallel beam test protocol is designed to evaluate the motor coordination of mice. This protocol is designed to assess motor skills, particularly in neurological diseases such as Parkinson's disease.
Light-dark box test for mice
By Emma Sherrell onThis protocol describes the setup and execution of the light-dark box test for mice, a behavioral experiment used to assess anxiety-related responses. The protocol involves preparing a light-dark box in a controlled environment, utilizing specific lighting conditions and camera settings for optimal observation and recording.
Rotarod test for mice
By Emma Sherrell onThe Rotarod test protocol for mice assesses motor coordination and balance. The test measures the latency to fall and speed at the fall.
Locomotion test for mice
By Emma Sherrell onThe locomotion test is an open field test in darkness, so it does not aim to measure anxiety, only locomotor activity.
Live tracking of multiple mice in a neuromelanin-inducing PD model over several weeks
By Emma Sherrell onThe Live Mouse Tracker (LMT) system is engineered for long-term, automated tracking and behavioral analysis of mice, integrating machine learning, computer vision, and RFID technology for simultaneous monitoring of multiple subjects.
Fontana-Masson staining
By Emma Sherrell onThe Fontana-Masson staining protocol is a detailed method for staining free-floating sections (20-50 microns) to detect melanin and other pigments in tissue samples.