Immunohistochemistry for carbon fiber thread electrodes
By Emma Sherrell onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By Emma Sherrell onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Human neuroblastoma cell line SH-SY5Y culturing
By Emma Sherrell onThis is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
Histology and retention of implanted carbon fiber thread electrodes in fixed tissue
By Emma Sherrell onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
Y-Maze protocol
By Julia Julia Leonard onThe Y-maze is a quick and easy way to evaluate a rodent’s spatial working memory by measuring its ability to effectively explore a Y-shape maze.
Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents
By Julia Julia Leonard onProtocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.
Immunostaining
By Julia Julia Leonard onThis is a protocol that describes how to use antibody staining to detect cellular epitopes by immunofluorescence microscopy.
Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
By Julia Julia Leonard onThis protocol describes an immunoprecipitation method to detect ubiquitination on a protein of interest.
Virus production and administration
By Julia Julia Leonard onProtocol for AAV production and administration to mice.
Tissue preparation, immunohistochemistry, imaging, and quantification
By Julia Julia Leonard onProtocol for neuronal imaging.
GCaMP6f fluorescence in ex vivo intestinal preparation
By Julia Julia Leonard onProtocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Metagenome preparation and analysis
By Julia Julia Leonard onProtocol for metagenomic analysis from mouse fecal pellets.
Expansion microscopy with R1441C LRRK2 MEF cells: Visualization of Myc-RILPL1 and TMEM55B
By Emma Sherrell onExpansion microscopy is a super-resolution imaging technique that uses expandable hydrogels to increase the physical distance between fluorophores from fixed cells on coverslips. By isotropically expanding samples, the traditional wavelength-limited confocal microscopy imaging techniques can be improved upon ~4X in resolution. Here, the authors use expansion techniques developed previously by others to confocal image TMEM55B and RILPL1.
Determination of NM concentration
By Emma Sherrell onThis is the protocol for determining neuromelanin concentration and data.
Metabolomics preparation and analysis
By Julia Julia Leonard onProtocol for metabolomic analysis from mouse fecal and cecal samples.
Chemical degradation and determination of pheomelanin and eumelanin markers
By Emma Sherrell onThis is a protocol involving chemical oxidation and reduction methods followed by high-performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.
Proteome preparation and analysis
By Julia Julia Leonard onProtocol for proteomic analysis from mouse cecal samples.
3′ mRNA-sequencing
By Julia Julia Leonard onProtocol for 3' mRNA sequencing of mouse gastrointestinal tract tissues.
Whole gut transit time, fecal water content, and fecal output
By Julia Julia Leonard onProtocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output).
Characterization of splay characteristics of carbon fiber electrode threads (CFETs) in agar brain phantom
By Emma Sherrell onMethods to measure deflection characteristics of carbon fiber electrodes in agar brain phantoms.