Protein structure prediction with AlphaFold-Multimer
By savannah onThis protocol describes the protein structure prediction with AlphaFold-Multimer.
Kinase activity assays Src and CK2
By savannah onThis protocol details about the Kinase activity assays of Src and CK2.
Purification mCherry-ATG13 IDR
By savannah onThis protocol describes how to express and purify mCherry-ATG13 IDR.
Purification GFP-ATG13 IDR
By savannah onThis protocol describes how to express and purify GFP-ATG13 IDR.
Luminex-based multiplex cytokine analysis protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onLuminex-based multiplex cytokine analysis protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Gene expression protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onGene expression protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Experimental design protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onExperimental design protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Ex vivo culture of SPMs protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onEx vivo culture of SPMs protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Animal housing and ethical concerns protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onAnimal housing and ethical concerns protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Rotarod test protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onRotarod test protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
IHC and image analysis related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onIHC and image analysis related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Tissue and blood collection related to “LRRK2-G2019S Synergizes with Aging and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onTissue and blood collection related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Western blot analysis V.1 used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onWestern blot analysis V.1 used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Primary glia isolation and culture protocol
By Emma Sherrell onThis protocol details the isolation and maintenance of microglia, oligodendrocytes, and astrocytes derived from early postnatal primary mouse cortex.
Magic red cathepsin B activity assay
By Emma Sherrell onThis protocol details the magic red cathepsin B activity assay for cultured cells.
Statistical analysis protocol for “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onStatistical analysis protocol for "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Lysosomal isolation protocol
By Emma Sherrell onThis protocol details the isolation of lysosomes from in vitro cultured cells.
List of IHC/WB antibodies, RT-PCR probes, and software used
By savannah onList of IHC/WB antibodies, RT-PCR probes, and software used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
