Generation of knockout iPSCs using CRISPR-Cas9 genome editing

By Nisha Mohd Rafiq, PhD and Pietro De Camilli, PhD
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Output Details

Description
This protocol describes the genetic modification of induced pluripotent cells (iPSCs) using CRISPR-Cas9, including synthesis of gRNA plasmids, transfection, selection of clones, and sequencing of genomic DNA to confirm knockout generation. The steps described in this protocol is based on Skarnes et al. (2019) and Fernandopulle et al. (2018).
Identifier (DOI)
10.17504/protocols.io.36wgqnr33gk5/v1
Team
Team De Camilli
Program
Collaborative Research Network
Theme
PD Functional Genomics

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