Imaging single SYTOX Orange molecules on a PLL-coated cover glass V.1

This is a protocol for the preparation of a microscopy sample of single SYTOX Orange molecules on a PLL-coated cover glass. This protocol was used to generate the data shown in Figure 1a, 1b and 1c of the following publication: Bruggeman et al., POLCAM: Instant molecular orientation microscopy for the life sciences. bioRxiv 2023.02.07.527479 (Feb 2023), doi: https://doi.org/10.1101/2023.02.07.527479