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A human development-based protocol for the differentiation of human ESCs into midbrain dopaminergic neurons.
Output Details
Description
Protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons. This protocol recapitulates the activation of developmental pathways known to be important for mDA neuron development and mimics key sequence of events taking place in human midbrain development.
The protocol involves activation of not only Shh and WNT/b-catenin signaling, but also the sequential activation of additional pathways that have not been previously implemented in hESC differentiation, including: activation of non-canonical WNT signaling by WNT5A, of integrin/YAP signaling by Laminin 511, and of the nuclear receptors NR1H3 and NR1H2 by GW3965.