Fura-2 a.m. imaging in hiPSCs for measuring intracellular calcium

By Peter kilfeather
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Output Details

Description
This protocol details steps for Fura-2 imaging to measure cytoplasmic calcium in hiPSCs treated with R568 (10 μM), a positive allosteric modulator of CASR. The cells were stimulated with ionomycin (5 μM) in order to estimate the levels of calcium stored in the intracellular compartments.
Identifier (DOI)
10.17504/protocols.io.kqdg39p4zg25/v1
Tags
  • Calcium
  • Confocal microscopy
  • hiPSCs (Human induced pluripotent stem cells)
  • Human
  • In Vitro
  • Microscopy - optical
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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    Peter kilfeather

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