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Analysis of glycosphingolipids from human cerebrospinal fluid
Output Details
Description
We have updated and refined the GSL analysis protocol with the focus on achieving sensitive and reproducible quantitation of GSLs in control and patient cerebrospinal fluid samples. The method uses the fluorescent compound anthranilic acid (2-AA) to label oligosaccharides prior to analysis using normal-phase high-performance liquid chromatography. The labelling procedure is rapid, selective, and easy to perform. With the inclusion of a 2AA-labelled chitotriose calibration standard, it is possible to obtain accurate and reproducible molar quantities of individual GSL species.
Identifier (DOI)
10.17504/protocols.io.5qpvorrndv4o/v1