Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs

By Oriol Busquets Figueras, PhD, Hanqin Li, PhD, Steven Poser, PhD, Frank Soldner, MD and Dirk Hockemeyer, PhD
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Output Details

Description

This collection describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection or electroporation.

Identifier (DOI)
10.17504/protocols.io.e6nvwkkewvmk/v2
Tags
  • Cell culture
  • Electroporation
  • FACS
  • hPSCs (Human pluripotent stem cells)
  • iPSCs (Induced pluripotent stem cells)
  • Nucleofection
Team
Team Rio
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Oriol Busquets Figueras, PhD

    Key Personnel: Team Rio, 

    Albert Einstein College of Medicine

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    Hanqin Li, PhD

    Key Personnel: Team Rio, 

    University of California, Berkeley

  • Steven Poser, PhD

    Key Personnel: Team Rio, 

    University of Washington

  • Frank Soldner, MD

    Co-PI (Core Leadership): Team Rio, 

    Albert Einstein College of Medicine

  • Dirk Hockemeyer, PhD

    Co-PI (Core Leadership): Team Rio, 

    University of California, Berkeley

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