RNAScope in situ hybridization (ISH) multiplex fluorescent

By Roberta Marongiu, PhD and Santiago Unda, MD
View Published Protocol

Output Details

Description
Instructions to perform ISH using RNAscope kit from ACDBio company optimized for frozen mouse brain and nodose ganglia sections.
Identifier (DOI)
10.17504/protocols.io.81wgb67o1lpk/v1
Tags
  • in situ RNA analytics
  • Other
Team
Team Kaplitt
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

In Situ Hybridization -Mendelsohn et al 2025

This protocol outlines the steps for preparing mouse brain tissue for In Situ Hybridization from Mendelsohn et al 2025.

View Protocol

RNA In situ hybridization (ISH) using RNAscope®

This protocol outlines the procedures for RNA in situ hybridization to simultaneously detect up to three targets using the RNAscope® Multiplex Fluorescent v2 assay (ACD).

View Protocol

L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)

Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).

View Lab Material
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.