EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)

By Elisabeth Holzer, MSc
View Published Protocol

Output Details

Description
This protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP. The expression is performed with E. coli Rosetta pLysS cells. The protein is purified via a GST batch purification and gel filtration (SEC).
Identifier (DOI)
10.17504/protocols.io.261gedpojv47/v1
Tags
  • Protein purification
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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    Elisabeth Holzer, MSc

    Key Personnel: Team Hurley, 

    Max Perutz Labs

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