Pipeline for Image Processing and Quantification of Striatal Dopamine Innervation using Fiji (IF/IHC)

Output Details

Method for quantifying striatal innervation by measuring mean fluorescence intensity using ImageJ. This protocol outlines the image preprocessing steps required prior to analysis with ImageJ tools as well as the ImageJ pipeline to quantify striatal innervation. Here DAT and TH immunofluorescence coronal sections but this method can be adapted for other markers and XYZ planes.

Meet the Authors

  • Natalia Lopez Gonzalez del Rey, PhD

    Key Personnel: Team Awatramani

    Northwestern University

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    James Lyman, BSc

    Key Personnel: Team Awatramani

    Northwestern University

Aligning Science Across Parkinson's
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