Miniscope calcium imaging data acquisition of cortical activity in non-human primates (NHPs)

In recent years, calcium imaging has become a standard technique in experiments utilizing rodent experimentation and is now also becoming useful in primate research. The size of the primate brain precludes the use of this technique to monitor deep brain structures in monkeys, and the relatively low density and substantial brain movement between experiments in NHPs complicate this research. Nevertheless, the ability to follow an indicator of activity in multiple simultaneously recorded and genetically defined neurons with sub-second time resolution is highly attractive and bound to lead to important scientific insights. Below, we briefly describe the essential steps in our laboratory’s calcium imaging experiments.