New micro-fiber array allows chronic measurement and optogenetic manipulation at 100+ locations in mice, for studying cell-type and neurotransmitter-specific signals in 3-D volumes. Protocol involves micro-CT scanning and fiber localization.
Protocol details surgical steps for viral injections and optical fiber implantation in mouse striatum to monitor neurotransmitter dynamics during learning and extinction, using genetic sensors and tetanus toxin to suppress acetylcholine release.
