Confocal microscopy to assess TelC and iGluSnFR expression in fixed mouse brain slices

By Safa Bouabid, PhD and Mark Howe, PhD
View Published Protocol

Output Details

Description
This protocol describes the tissue processing steps following (a) glutamate recordings using the genetically encoded glutamate sensor (iGluSnFR) and (b) behaviour experiments following tetanus toxin light chain (TelC) viral injection in the dorsal medial striatum.
Tags
  • Confocal microscopy
  • Glutamate signaling
  • Microscopy - optical
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations

Striatal acetylcholine (ACh) signaling in mice shows opposing changes across different regions, with positive and negative prediction errors encoded in anterior dorsal striatum.

View Article

Source data for Motor Cortical Neuronal Hyperexcitability Associated with α-Synuclein Aggregation

Chen et al (2024) study links α-synuclein aggregation to hyperexcitability in motor cortical neurons.

View Dataset

Single-cell Whole Genome Amplification (scWGA) of human post-mortem brain samples isolated by Laser Capture Microdissection (LCM)

This protocol uses Laser Capture Microdissection (LCM) technology on human post-mortem brain tissue slides in order to do low coverage single-cell whole genome sequencing to detect mega-base somatic Copy Number Variations (CNVs).

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.