Inflamed Microglia like Macrophages in the Central Nervous System of Prodromal Parkinson′s Disease
By onWe investigated the role of inflammation in the pathogenesis of prodromal PD performing single-cell RNAseq analysis of CSF and blood from 111 individuals.
Supporting data for “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
By onData associated with “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
iPSC QC (Adami et al, 2025)
By onSummary table and data from iPSC quality control experiments in Adami et al. 2025 are accessible for review.
Stimulation of DA neurons and microglia
By onThis protocol details how to stimulate microglia with IFN-gamma, poly-IC and TNF-alpha.
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_LacZ)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and glial activation without dopamine neuron axon terminal loss
By onWe tested whether a gut infection causes blood-brain barrier permeability and brain inflammation. Results showed increased BBB permeability in specific brain regions at 26 days while tight-junction and dopamine related proteins remained unchanged
Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis
By onUsing single cell RNA sequencing, we demonstrate that LRRK2 G2019S is associated with an increased neutrophil presence in the colonic lamina propria during infection, Th17 skewing, upregulated Il17a, and greater colonic pathology.
LRRK2G2019S acts as a dominant interfering mutant in the context of iron overload
By onUsing a model macrophage cell line we showed that LRRK2-G2019S mutation affects iron homeostasis by dysregulating iron-related proteins and blocking ferritinophagy in iron overload conditions, suggesting a role in iron dysregulation in PD.
AutoMorphoTrack: An Automated Python Package for Organelle Morphology, Motility, and Colocalization Analysis in Live-Cell Imaging
By onAutoMorphoTrack is a Python package for analyzing organelle dynamics in live-cell imaging data. It automates detection, classification, tracking, and colocalization of mitochondria and lysosomes, enabling reproducible and quantitative analysis.
