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DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
onThis protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
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Data and images for Tonic dendritic GABA release by substantia nigra dopaminergic neurons
onDatasets from Simmons et al 2024.
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Immunohistochemistry for brain sections
onProtocol for processing 30um mouse brain sections for immunolabeling.
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Nova-ST Spatial Transcriptomics protocol
onNova-ST is an open-source, high-resolution sequencing-based spatial transcriptomics workflow. This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq. Nova-ST is derived from dense nano-patterned randomly barcoded Illumina NovaSeq 6000 S4 sequencing flow cells.
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Nuclei isolation, immunostaining, and Fluorescence-activated nuclei sorting (FANS) for Smart-Seq2
onThis protocol describes the steps for extracting nuclei from human postmortem brain samples, immunofluorescence, and nuclei sorting (FANS) for single-nucleus RNA-seq using the Smart-Seq2 method.
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Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS)
onThis protocol describes the isolation steps of nuclei from human post-mortem brain samples, immunofluorescence, and nuclei sorting (FANS) for low coverage (<1x) single-cell Whole Genome Sequencing (scWGS) to detect mega-base somatic Copy Number Variations (CNVs).
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The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons
onIn this study, the authors utilized human and murine neuronal lines, stem cell-derived dopaminergic neurons, and mice to demonstrate that three previously identified genetic risk factors for PD, namely SATB1, MIR22HG, and GBA, are components of a single gene regulatory pathway.
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TNF-NF-κB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons
onThe authors identified p53-mediated apoptotic cell death as a major contributor to dopamine neuron loss and uncovered a causal link of TNFa-NFκB signaling in limiting cell survival.
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Saliva metagenomic data related to “Evaluation of an adapted semi-automated DNA extraction for human salivary shotgun metagenomics”
onIn this study, the authors proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer.
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scNAT Data for “scNAT: A deep learning method for integrating paired single-cell RNA and T cell receptor sequencing profiles”
onData for "scNAT: A deep learning method for integrating paired single-cell RNA and T cell receptor sequencing profiles" publication.
