AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque

By Miguel Chuapoco
View Published Protocol

Output Details

Description
This protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Identifier (DOI)
10.17504/protocols.io.3byl4jo68lo5/v1
Tags
  • AAV (Adenoassociated Virus)
  • In Vivo
  • Non-human primate
  • Postmortem
Team
Team Gradinaru
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Miguel Chuapoco

    Key Personnel: Team Gradinaru, 

    California Institute of Technology

Related Research

Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain

Crossing the blood–brain barrier in primates is a major obstacle for gene delivery to the brain. Adeno-associated viruses (AAVs) promise robust, non-invasive gene delivery from the bloodstream to the brain. However, unlike in rodents, few…

View Article

pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

View Lab Material

Ef1a-DIO-PSAML-141F,Y115F-GlyR-2A-FusionRed plasmid

Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

View Lab Material
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.