Axonal proteome analysis and Western Blot via APEX2 labeling
Output Details
Description
We and others previously reported that knock-in mice expressing the pathogenic hyperactive Lrrk2G2019S mutation, exhibit deficits in dopamine release within the striatum. To investigate the underlying molecular mechanisms, we conducted axonal proteome analysis of a subcluster within vulnerable Aldh1a1+ dopamine axons, defined by Annexin 1 (Anxa1+), confirmed subtle proteomic changes across comparisons.
Identifier (DOI)
10.17504/protocols.io.n2bvjb79bgk5/v1
