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Chronic optrode recordings from the Locus coeruleus (Optrode construction, implantation, and recording)
Output Details
Description
This protocol covers chronic optrode construction, implantation, and recording in the Locus coeruleus (LC). It begins with building the optrode, involving assembly of a plastic housing, screw, thumbnut, and electrode interface board (EIB-32), followed by attaching electrodes and optic fibers to the EIB-32. The electrodes are then glued and trimmed, ensuring protection from animal-related damage.
Implantation involves anesthesia, craniotomy, and careful electrode positioning. A gradual approach is recommended for electrode insertion to minimize tissue damage. The optrode is secured with cement and vaseline for stability.
For recording from the LC, an OpenEphys system with omnetics headstage and acquisition software is used. Animals undergo 30-minute sessions with opto-tagging via red or blue light pulses. The setup ensures precise data acquisition and identification of neuron responses.
Identifier (DOI)
10.17504/protocols.io.rm7vzxd45gx1/v1