A macro that counts colocalized synaptic puncta in microscopy images. Analysis involves noise reduction, thresholding, puncta counting, and colocalization calculation.
QuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Extensive image processing techniques were used to analyze cellular mechanisms, including deconvolution, machine learning, and manual identification of structures. Creative approaches were employed to ensure reliable and reproducible results.
