Differentiation of WTC11 and KOLF2.1 iPSCs to dopaminergic neurons (forked)

By Nisha Mohd Rafiq, PhD and Pietro De Camilli, PhD
View Published Protocol

Output Details

Description
This protocol describes the differentiation of iPSCs (WTC11 and KOLF2.1) to dopaminergic neurons according to Bressan et al 2021.
Identifier (DOI)
10.17504/protocols.io.dm6gp39m8vzp/v1
Team
Team De Camilli
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary properties in iPSC-derived dopaminergic neurons

We show that a mutation in SJ1 that causes early-onset Parkinsonism affects the cilia of iPSC-derived DA neurons. Cilia are longer and show an accumulation of calcium channels and ubiquitinated proteins relative to control neurons, suggesting an effe

View Article

How Membrane Contact Sites Shape the Phagophore

Phagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.

View Article

FISH CODETECTION

DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections

View Protocol
View More Outputs