Ex vivo imaging of dendritic calcium transients and spines with two-photon laser scanning microscopy (2PLSM)

Output Details

This protocol is adapted from: [http://dx.doi.org/10.17504/protocols.io.n92ldry68g5b/v1](http://dx.doi.org/10.17504/protocols.io.n92ldry68g5b/v1)) and modified for imaging dendritic Ca2+ transients and dendritic spines of striatal spiny projection neurons (SPNs) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Tags
  • Ex Vivo
  • Microscopy - optical
  • Mouse
  • Two-Photon Microscopy

Meet the Authors

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    Enrico Zampese, PhD

    Key Personnel: Team Surmeier

    Northwestern University

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    Shenyu Zhai, PhD

    Key Personnel: Team Surmeier

    Northwestern University