Ex vivo imaging of dendritic calcium transients and spines with two-photon laser scanning microscopy (2PLSM)

By Enrico Zampese, PhD and Shenyu Zhai, PhD
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Output Details

Description
This protocol is adapted from: [http://dx.doi.org/10.17504/protocols.io.n92ldry68g5b/v1](http://dx.doi.org/10.17504/protocols.io.n92ldry68g5b/v1)) and modified for imaging dendritic Ca2+ transients and dendritic spines of striatal spiny projection neurons (SPNs) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Identifier (DOI)
10.17504/protocols.io.4r3l294nqv1y/v1
Tags
  • Ex Vivo
  • Microscopy - optical
  • Mouse
  • Two-Photon Microscopy
Team
Team Surmeier
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Enrico Zampese, PhD

    Key Personnel: Team Surmeier, 

    Northwestern University

  • User avatar fallback logo

    Shenyu Zhai, PhD

    Key Personnel: Team Surmeier, 

    Northwestern University

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