This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS)
Output Details
Description
This protocol describes the isolation steps of nuclei from human post-mortem brain samples, immunofluorescence, and nuclei sorting (FANS) for low coverage (<1x) single-cell Whole Genome Sequencing (scWGS) to detect mega-base somatic Copy Number Variations (CNVs). We have used this protocol to isolate nuclei from the frontal cortex, cingulate cortex, and substantia nigra tissues. However, it can be adapted for nuclei from different body areas, cell culture materials, and/or samples from other species.
Identifier (DOI)
10.17504/protocols.io.81wgbzybygpk/v1