Formation and isolation of Clu phospholipid particles

By Patricia Yuste Checa, PhD, Andreas Bracher and Franz-Ulrich Hartl, MD
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Output Details

Description
This protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).
Identifier (DOI)
10.17504/protocols.io.bp2l6x59zlqe/v1
Tags
  • CLU (Clusterin)
  • Isolation
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

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HEK293E_Clu WT

Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

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