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Generation of stable LysoTag expressing cell lines and LysoTag immunoprecipitation of lysosomes
Output Details
Description
Here we describe how to produce stable LysoTag expressing cell lines and how to perform rapid isolation of lysosomes in cultured cells with immunoprecipitation of the LysoTag using HA-coupled magnetic beads. The immunoprecipitation protocol is very fast, less than 15min from start of the incubation with the beads. The protocol can be used to immunoprecipitate lysosomes from commonly cultured cells such as mouse embryonic fibroblast, HEK293 and A549 cells expressing the LysoTag.
Identifier (DOI)
10.17504/protocols.io.261gedz2ov47/v1