Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are the most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell…
This protocol is used to label endogenous LRRK2 in mouse. It has been edited from West et al., 2014. Protocol optimized for fresh fixed brain tissue sectioned at 40 um.
