Immunological detection of APP and proteins of the endolysosomal system

Description

This protocol describes the immunological detection of APP and proteins of the endolysosomal system.

Here we present a general protocol for immunological detection by Western blotting of APP and proteins of the endolysosomal system, including EEA1, RAB5, PSEN1, LAMP1, LAMP2, TMEM192, and BACE1.

Identifier (DOI)

10.17504/protocols.io.kqdg36jeeg25/v2

Tags

Endolysosomes
Endosomes
Lysosomes
Western Blot

Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Hankum Park, PhD

Key Personnel: Team Harper,

Harvard University
Frances Hundley, PhD

Project Manager: Team Harper,

Harvard University
J. Wade Harper

Lead PI (Core Leadership): Team Harper,

Harvard University

Immunological detection of APP and proteins of the endolysosomal system

Output Details

Meet the Authors

Hankum Park, PhD

Frances Hundley, PhD

J. Wade Harper

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