Microendoscopic calcium imaging in motor cortices of macaques during rest and movement
Published June 20, 2025
Output Details
Published June 20, 2025
Description
The study of motor cortices in non-human primates is relevant to our understanding of human motor control, both in healthy conditions and in movement disorders. Calcium imaging and miniature microscopes allow the study of multiple genetically identified neurons with excellent spatial resolution. We used this method to examine activity patterns of projection neurons in deep layers of the supplementary motor (SMA) and primary motor areas (M1) in four rhesus macaques. We implanted gradient index lenses and expressed GCaMP6f to image calcium transients while the animals were at rest or engaged in an arm-reaching task. We tracked the activity of SMA and M1 neurons across conditions, examined cell pairs for synchronous activity, and assessed whether SMA and M1 neuronal activation followed specific sequential activation patterns. We demonstrate the value of *in vivo* calcium imaging for studying patterns of activity in groups of corticofugal neurons in SMA and M1.
Identifier (DOI)
10.1016/j.isci.2025.112767
