Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma

By Alexandra Kazanova, PhD
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Output Details

Description
This protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma. Previously, quantification of the number of IL-2 producing 2cz hybridoma cells in an ELISPOT assay was used as a readout for MitAP (Matheoud et al., 2016). Here, we adapted the assay for the detection of activation-induced markers (AIM) on 2cz cells with flow cytometry.
Identifier (DOI)
10.17504/protocols.io.5qpvok9j7l4o/v1
Tags
  • Activity Assay
  • Antigen-presenting cells (APCs)
  • Antigen-specific response
  • Mitochondrial
Team
Team Desjardins
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Alexandra Kazanova, PhD

    Key Personnel: Team Desjardins, 

    McGill University

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