The authors developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPRmts role in maintaining proteostasis during stress.
Study aimed to find proteins interacting with mitophagy receptors NIX or BNIP3 by incubating NIX-GST or BNIP3-GST with HeLa cell lysate to identify binding partners.