Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association

Output Details

This protocol outlines a dual-cue delay Pavlovian association and multi-fiber photometry recordings in head-fixed mice. Mice are first placed on a controlled water schedule and habituated to head fixation on a spherical treadmill. During the conditioning phase, they learn to associate visual and auditory cues with water rewards, followed by a partial extinction phase where reward probabilities are downshifted.
Tags
  • Acetylcholine
  • Behavioral
  • Dopamine
  • Mouse

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