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Neurite_FISH_Quant–Python code for quantification of FISH puncta in neurites
Output Details
Description
This repository contains Jupyter Notebooks with Python 3 code for quantification of FISH puncta within neuronal dendrites or axons. However, prior identification of FISH puncta in the images (and optional neurite segmentation) is required. We use ImageJ plugins for this purpose, as specified below. The workflow assumes Z-stack, multi-channel fluorescence images, with one channel used for identification of neurites and other channels for FISH.
Identifier (DOI)
10.5281/zenodo.5570748